A modular platform for bioluminescent RNA tracking

Abstract A complete understanding of RNA biology requires methods for tracking transcripts in vivo.Common strategies rely on fluorogenic probes that are limited in sensitivity, dynamic range, VIBRATOR RABBIT/DUAL STIM and depth of interrogation, owing to their need for excitation light and tissue autofluorescence.To overcome these challenges, we re

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Transcript Profiles of Blumeria graminis Development During Infection Reveal a Cluster of Genes That Are Potential Virulence Determinants

High-density cDNA microarrays (2,027 unigenes) were used to analyze transcript profiles of Sensor EXT Wire the plant-pathogenic fungus Blumeria graminis f.sp.hordei throughout its asexual life cycle and development of infection.RNA was obtained from four stages preceding penetration and four stages after penetration of the host cells.The microarray

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