Abstract A complete understanding of RNA biology requires methods for tracking transcripts in vivo.Common strategies rely on fluorogenic probes that are limited in sensitivity, dynamic range, VIBRATOR RABBIT/DUAL STIM and depth of interrogation, owing to their need for excitation light and tissue autofluorescence.To overcome these challenges, we report a bioluminescent platform for serial imaging of RNAs.
The RNA tags are engineered to recruit light-emitting luciferase fragments (termed RNA lanterns) upon transcription.Robust photon production is observed for RNA targets both in cells and in live animals.Importantly, only a single copy of the tag is necessary for sensitive detection, in sharp contrast to fluorescent platforms requiring multiple repeats.
Overall, Commercial Convection Ovens this work provides a foundational platform for visualizing RNA dynamics from the micro to the macro scale.